Author:
Yung-ding Bow#, Yen-yun Wang#, Yuk-kwan Chen, Chang-wei Su, Ching-wei Hsu, Ling-yi Xiao, Shyng-shiou Yuan*, and Ruei-nian Li*
Abstrast:
Objectives
FOXA2 gene methylation links to the progression of cancers, but has not been documented in oral cancer. Herein, we explore the role of FOXA2 in the migration of oral cancer cells.
Material and Methods
Methylation‐specific PCR was applied for gene methylation. Wound healing and transwell experiments were tested for cell migration. FOXA2 expression in oral cancer tissues was addressed by immunohistochemistry, followed by statistical analysis of its association with clinical manifestations and patient survival.
Results
FOXA2 bound to the promoter of CDH1 and enhanced the expression of its gene product E‐cadherin, and decreased the cancer cell migration activity. High FOXA2 expression in oral cancer tissues was associated with high E‐cadherin expression, decreased lymph node metastasis, and increased patient survival.
Conclusion
FOXA2‐E‐cadherin link is involved in regulation of oral cancer cell metastasis and provides a new insight for the tumor suppressor activity of FOXA2 in oral cancer.
Key word:
gene methylation; oral cancer; cell migration; FOXA2; E-cadherin